Sodium in PDB 7pfr: Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus

All present enzymatic activity of Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus:
1.10.3.2;

The structure of Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus, PDB code: 7pfr was solved by A.Gabdulkhakov, S.Tishchenko, I.Kolyadenko, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.24 / 1.75
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	74.98, 94.12, 119.73, 90, 91.16, 90
R / Rfree (%)	13.1 / 16.9

The structure of Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus also contains other interesting chemical elements:

Copper

(Cu)

24 atoms

The binding sites of Sodium atom in the Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus (pdb code 7pfr). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus, PDB code: 7pfr:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus within 5.0Å range: probe atom residue distance (Å) B Occ A:Na405 b:44.7 occ:1.00 O C:HOH615 2.4 32.2 1.0 O B:HOH542 2.4 34.6 1.0 O A:HOH623 2.5 40.1 1.0 O B:HOH526 2.5 33.3 1.0 O C:HOH527 2.5 37.6 1.0 O A:HOH554 2.6 32.5 1.0 O A:HOH672 3.4 48.4 1.0 O C:HOH565 3.8 29.4 1.0 O A:HOH597 3.8 33.6 1.0 O B:HOH553 3.9 28.0 1.0 O C:ASP243 4.3 27.5 1.0 O A:ASP243 4.4 28.0 1.0 O B:ASP243 4.4 27.2 1.0 OG C:SER256 4.5 28.0 1.0 OG A:SER256 4.5 27.2 1.0 OG B:SER256 4.6 27.5 1.0 O B:HOH574 4.9 56.0 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Two-Domain Laccase Mutant M199A From Streptomyces Griseoflavus within 5.0Å range: probe atom residue distance (Å) B Occ D:Na404 b:48.1 occ:1.00 O F:HOH644 2.4 31.1 1.0 O E:HOH605 2.5 33.8 1.0 O D:HOH530 2.5 31.4 1.0 O D:HOH539 2.5 33.3 1.0 O E:HOH524 2.6 37.4 1.0 O F:HOH544 2.6 35.6 1.0 O D:HOH648 3.5 44.2 1.0 O D:HOH574 3.7 34.8 1.0 O E:HOH578 3.9 30.7 1.0 O F:HOH589 3.9 34.8 1.0 O D:ASP243 4.3 27.8 1.0 O E:ASP243 4.3 25.7 1.0 O F:ASP243 4.4 24.6 1.0 OG D:SER256 4.5 27.5 1.0 OG F:SER256 4.6 26.3 1.0 OG E:SER256 4.7 29.5 1.0 CA D:SER256 5.0 23.8 1.0

I.Kolyadenko, A.Scherbakova, K.Kovalev, A.Gabdulkhakov, S.Tishchenko. Engineering the Catalytic Properties of Two-Domain Laccase From Streptomyces Griseoflavus Ac-993. Int J Mol Sci V. 23 2021.
ISSN: ESSN 1422-0067
PubMed: 35008493
DOI: 10.3390/IJMS23010065